Isolation and characterization of microsatellites in Chinese white olive (Canarium album) and cross-species amplification in Canarium pimela

Abstract

We developed 11 polymorphic microsatellite primers from Canarium album, which is a famous Chinese fruit tree with diversified economy values. The observed and expected heterozygosities (Ho and He) ranged from 0.133 to 0.833 and 0.128 to 0.810, with averages of 0.607 and 0.629, respectively. Four loci were deviated from Hardy–Weinberg equilibrium after Bonferroni correction (P < 0.01), but no linkage disequilibrium was detected among any pair of loci after Bonferroni correction (P < 0.05). Cross-species amplification in another important cultured species Canarium pimela showed that 10 of 11 primers were polymorphic, with number of alleles per locus ranging from 2 to 5. The primers will be useful to explore further studies about introgressive hybridization and conversation of wild genetic resources of these two species.