Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification

Abstract

Garcinia indica popularly known as ‘Kokum’ or Murugalu”, is a medium-sized evergreen tree found in the western-ghats of India. This tree species is highly exploited to produce anti-obesity drugs and culinary purposes. Its population is threatened by overexploitation and loss of habitat. The development of microsatellite markers would help in understanding the genetic structure and further to develop appropriate conservation strategies. In this study, using next generation sequencing platform Illumina Hiseq 2000, we have sequenced the partial genome of G. indica and identified 3725 microsatellites. Forty-eight microsatellite markers were analyzed using 30 accessions. Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922. Allele per locus ranged from 3 to 33 per locus. The probability of identity values ranged from 0.00329 to 0.30489. Cross-species amplification SSR primers in the related species showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)