Abstract
Intertidal snails of the genus Batillaria are a common group in tidelands throughout Japan. Recently, population sizes of Batillaria multiformis have been reduced due to coastal area reclamation. Using next generation sequencing, we developed 11 microsatellite markers to reveal the genetic diversity and gene flow among populations and combined them into a single multiplex panel. Evaluating polymorphisms in two populations showed that the numbers of alleles per locus ranged from 2 to 17 and 2 to 15, and the expected heterozygosity ranged from 0.274 to 0.936 and 0.455 to 0.934 in Mangokuura estuary and Kasari Bay, respectively.
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