Abstract
Tachypleus tridentatus is an endangered horseshoe crab that is found and known only in several isolated sites in southern China, and that has been suffering from the loss and fragmentation of its habitat. To evaluate its genetic diversity and population genetics, the first set of eight polymorphic microsatellite loci was developed and characterized. The number of alleles ranged from 3 to 18 per locus with the observed and expected heterozygosities ranging from 0.667 to 1.000 and from 0.543 to 0.944, respectively. These markers will be useful for the assessment of genetic variation for this species.
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