Abstract
A Crotalus horridus genomic library was screened for clones containing microsatellite loci by hybridization with oligonucleotides consisting of a (dC x dA)n dinucleotide repeat. Primers designed to amplify six of the microsatellite loci were used to screen 32 unrelated individuals representing populations in eastern Pennsylvania, southern New Jersey, North Carolina, South Carolina, and Alabama. The six microsatellite loci were all polymorphic, with two to nine alleles, and heterozygote frequencies at each locus from 0.1 to 0.69. Allelic frequencies varied among geographically separated populations. Screening of two families produced no evidence of multiple paternity. These microsatellite markers should be useful for the assessment of kinship relationships and genetic diversity within and between populations of C. horridus. The application of this technology could provide a valuable tool for the development of effective conservation and management programs for threatened and endangered populations.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
