Abstract
This paper describes the characterization of a set of nine microsatellite markers for the pipe-organ mud-dauber wasp, Trypoxylon albitarse F. (Hymenoptera: Crabronidae), a common solitary wasp species in Brazil. Eight of the nine loci described were found to be polymorphic. The number of alleles ranged from three to 11. Observed and expected heterozygosity on the population level ranged from 0.135 to 0.891 and 0.177 to 0.865, respectively. Genotypic analyses revealed no deviations from Hardy-Weinberg equilibrium or linkage disequilibrium after sequential Bonferroni correction. Cross-species amplification was also tested for nine related species and positive amplifications were found in most of the assays. These markers will be useful in studies involving relatedness analysis and investigations of the population genetics of these wasps.
Highlights
Trypoxylon (Hymenoptera: Crabronidae) is a diverse genus of solitary wasps
The aim of the present study was to characterize microsatellite loci for the pipe-organ mud-dauber wasp, T. albitarse F., a common solitary wasp species in Brazil, the nests of which are often found in the walls of man-made constructions
Fragments between 400 and 1000 bp were purified from the gel (Wizard SV Gel and Polymerase chain reactions (PCRs) Clean-Up System kit, Promega)
Summary
Trypoxylon (Hymenoptera: Crabronidae) is a diverse genus of solitary wasps. Species of the subgenus Trypargilum appear to have several distinctive features, such as nest architecture, cocoon morphology and prey preference (Coville, 1982). It was necessary to develop specific markers for Trypoxylon, which might prove useful in familial and population genetics studies and provide valuable insights regarding the mating system of these wasps. The aim of the present study was to characterize microsatellite loci for the pipe-organ mud-dauber wasp, T. albitarse F., a common solitary wasp species in Brazil, the nests of which are often found in the walls of man-made constructions.
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