Isolation and characterization of microplastics from skin care products; interactions with albumin proteins and in-vivo toxicity studies on Artemia salina

Abstract

Skincare products are a significant source of primary microplastics (MPs). This study isolates and characterizes microplastic from two skin care products: face wash (FW-MPs) and face scrub (FS-MPs). Microplastics extracted were around 660 µm in size. The extracted MPs, designated as unground MPs (UG-MPs), were smooth surface and spherical. Ground ones were denoted as the ground MPs (G-MPs) that varied in size and surface shape. G-MPs interacted with bovine serum albumin (BSA) and human serum albumin (HSA). BSA adsorption on FW-MPs was 29%, whereas HSA adsorption was 47%. Contrarily, FS-MPs displayed 17% and 31%. Fluorescence spectroscopy and FE-SEM images showed HSA adsorption on G-MPs was greater than BSA. G-MP interaction changed the life cycle of Artemia salina. UV-Vis and fluorescence spectroscopy were used to study protein adsorption and desorption on G-MPs. A. salina treated to 2.5 mg/mL G-MPs delayed hatching and development and internalized microplastics in the gut at 144 h exposure.