Abstract
Numerous microbodies developed at the logarithmic phase of growth in cells of Candida tropicalis, when cultures were grown in a medium containing normal alkanes. From the homogenate of protoplasts prepared from 16-hr cells of Candida, biologically active microbodies were isolated by discontinuous sucrose density gradient centrifugation. The fractions recovered in the middle and lower-density regions of the gradient were associated with substantially high activities of three peroxisomal marker enzymes, catalase, D-amino acid oxidase, and urate oxidase, while a mitochondrial marker enzyme, cytochrome oxidase, was only slightly detected. Electron microscopic studies revealed that these fractions were largely comprised of morphologically well-preserved microbodies with a smaller number of more or less degraded organelles, but were virtually devoid of mitochondria. The microbody fractions contained an appreciable amount of DNA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
