Abstract
In this study, we isolated Bacillus licheniformis MH48 from rhizosphere soil and demonstrated that this strain shows significant antifungal activity against Rhizoctonia solani, Colletotrichum gloeosporioides, and Phytophthora capsici. Our results showed that a 50% concentration of bacterial cell-free culture filtrate of B.licheniformis MH48 shows strong activity against fungal pathogens. Benzoic acid produced by B.licheniformis MH48 was purified by various chromatographic techniques and identified by nuclear magnetic resonance and gas chromatography-mass spectrometry analysis. Benzoic acid displayed antifungal activity against R.solani and C.gloeosporides with minimum inhibitory concentration of 128μg/mL against mycelial growth. Microscopic examination revealed that benzoic acid (50μg/mL and 100μg/mL) transformed C.gloeosporioides conidial morphology and inhibited conidial germination. In addition, benzoic acid (100μg/mL and 200μg/mL) degraded R.solani mycelia. Therefore, our results demonstrate that B.licheniformis MH48 strain shows potential for utility as a biological agent for the control of various fungal pathogens of plants.
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