Abstract
To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.
Highlights
At first, studies on stem cells were restricted to progenitor cells committed to tissue renovation and differentiation, from resident cells in the same tissue[1,2,3]
Studies are preliminary concerning the ability of stem cells to originate cells with a mature phenotype different from that observed in the tissue of origin[10], they probably are the basis to the progress already seen in research associated to stem cell therapy[11,12], as an alternative to intermediate mechanisms responsible for the regeneration of diverse tissues
For the isolation method used to obtain bone marrow stem cells, our results were similar to Friedenstein et al.[28], when they established the ability to adhere to plastic as a main feature, when suspension cells are washed two to four times a day, after the beginning of the culture
Summary
Studies on stem cells were restricted to progenitor cells committed to tissue renovation and differentiation, from resident cells in the same tissue[1,2,3]. There are numerous animal models, such as rodents[12,15], swine[16] and canines[17,18,19] that have been used in experiments related to cell therapies. Regarding such animals, outcomes were found in studies about neurodegenerative injuries[12,13], as well as in attempts to use cell therapy as a way to treat cardiovascular diseases[20]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
