Isolation and Characterization of Lupeol from the Stem of Tapinanthus globiferus (A Rich.) and its Antimicrobial Assay

V Emaikwu,J.V Anyam,Ranj Hikmat Mohammed ,Ig Ndukwe ,Ogunkemi Risikat Agbeke Iyun

doi:10.4314/jasem.v24i6.11

Abstract

Lupeol, a pentacyclic triterpenoid, was isolated from hexane and ethyl acetate solvent system. In antiquity, the stem and leaf infusion of Tapinanthus globiferus has been used ethno-medicinally as a remedy for stomach ache, diarrhea, dysentery, and wounds. Lupeol isolation from this species was carried out by column chromatography after concentrating the crude extract using a rotary evaporator, and the structure was determined by analysis of the isolate by IR, 13CNMR, 1HNMR, HSQC, and HMBC spectral analysis as well as comparison with reported data. This is the first isolation of lupeol from the stem of this species.  Keywords: Tapinanthus globiferus, Column chromatography, dysentery, Lupeol

Highlights

Tapinanthus globiferus is a mistletoe of the family Loranthaceae
The objective of this paper is to report the isolation of lupeol from hexane-ethyl acetate extracts of birdlime (Tapinanthus globiferus) because Lupeol has demonstrated several intriguing pharmacological activities including anti-angiogenic, anti-microbial, anti-proliferative, anti-inflammatory, and cholesterollowering (Avin et al.,2014; Saleem, 2009; Gallo and Sarachine, 2009)
Isolation and Purification: The hexane and ethyl acetate extract of Tapinanthus globiferus were dissolved in a minimum amount of ethyl acetate and hexane solvent system 9:1, preabsorbed in silica gel and subsequently loaded onto a column packed with silica gel

Summary

Introduction

Tapinanthus globiferus is a mistletoe of the family Loranthaceae. It grows parasitically on trees and shrubs, most the Loranthaceae family grow in the tropics. It is a woody, spreading shrub with blackish, smooth stems that look rough due to lenticels. The previous studies conducted which includes antibacterial effect (Emaikwu et al, 2019) antioxidant effect (Cork et al, 1998) and antihypertensive studies via reduction of LDL and triglycerides. The objective of this paper is to report the isolation of lupeol from hexane-ethyl acetate extracts of birdlime (Tapinanthus globiferus) because Lupeol has demonstrated several intriguing pharmacological activities including anti-angiogenic, anti-microbial, anti-proliferative, anti-inflammatory, and cholesterollowering (Avin et al.,2014; Saleem, 2009; Gallo and Sarachine, 2009). Owing to its non-toxicity to normal cells and tissues, chemoprevention with lupeol is relatively a new avenue of oncology (Chaturvedi, 2008; Palanimuthu et al, 2012)

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