ISOLATION AND CHARACTERIZATION OF LIPASE PRODUCING BACTERIA FROM ANIMAL FATS

Abstract

Lipases are enzymes which have numerous applications in many industries like leather, soaps and detergents, pharmaceuticals, biofuel, food, textile etc. Many organisms like plants, fungi, bacteria are known to produce lipase. Lipolytic bacteria were isolated from Egyptian animal fats and grown on tributyrin media. Among the isolates from Egyptian animal fats three strains identified to be Lactobacillus suebicus DSM 5008 DSM, Pseudomonas oryzihabitans DSM 6835 THAMand Staphylococcus epidermidis 4b_r ESL. These strains were evaluated for potential probiotic properties. Absence of haemolytic activity and antibiotic resistance is considered as a safety prerequisite for the selection of a probiotic strains. One strain was γ-haemolytic (i.e. no haemolysis) and other were α-hemolysis. All of the strains were recorded highly lipolytic activity on Tributyrin agar base plates The highest lipase activity (651.9 µg/ml/min) was observed in Pseudomonas oryzihabitans DSM 6835 THAM but the lowest activity was recorded in Staphylococcus epidermidis 4b_r ESL(151.3 µg/ml/ min). On the other hand, Lactobacillus suebicus DSM 5008 DSM tested gave (337.9 µg/ml/min).