Abstract
ObjectivesThe biochemical characteristics of the inhibitory compound to lipase isolated from potato was compared to the commercially available anti-obesity drug, Orlistat. MethodsLipase inhibitory compound was isolated from the water extract from potato by serial solvent extraction and chromatographic purifications. The lipase inhibition kinetics was assessed by double reciprocal Lineweaver-Burk plotting. the potency of lipase inhibition of the compound was compared with Orlistat using 4-MUO as a substrate. ResultsThe lipase inhibitroy compound exhibited mixed type inhibition and high potency with an estimated IC50 of 216 ng/mL in the impure extract. The lipase inhibition activity of the compound is comparable to the commercially available anti-obesity drug, Orlistat, whose IC 50 was 0.4 ng/mL. The compound is stable at low pH (pH 2) and at boiling temperature for 1 h. Storage stability test showed that the compound is stable at least 4 weeks at RT. ConclusionsRegarding the stability, solubility in water and lipase inhibition activity, the compound isolated from potato has a potential to be developed as a safe anti-obesity remedy. Funding SourcesThis study was funded by a basic research grant from Korean National Research Fund.
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