Isolation and characterization of lignin-degrading bacterium Bacillus aryabhattai from pulp and paper mill wastewater and evaluation of its lignin-degrading potential.

Abstract

This study reports the degradation and decolourization capability of a manganese peroxidase enzyme producing bacterium isolated from pulp and paper mill wastewater. The isolate was identified as Bacillus aryabhattai based on biochemical analysis and 16S rRNA gene sequencing. The strain was designated MG966493. This bacterium was able to reduce 67% and 54% colour and lignin, respectively, from the pulp and paper mill wastewater after 144h of treatment at 32°C, pH 7.6 and 120rpm. Further, FT-IR analysis showed that during the lignin degradation process a number of metabolites were produced comprising different functional groups such as carbonyl (C=C), carboxyl (-COOH), alkene (C=C), amines (-NH2), sulphonic (-SO3) and nitro (-NO2). In addition, the SEM analysis showed that the bacterial cells exposed to pulp and paper mill wastewater have rough surfaces with reduced size as compared to the unexposed cells with smooth surfaces. This study concluded that the isolated bacterium B. aryabhattai has significant potential for the bioremediation of pulp and paper mill wastewater and thus, can be applied for their treatment at an industrial scale.