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Abstract
Prebiotics are good coumpounds used in the growth of lactic acid bacteria (LAB) in digestion, prebiotics come from vegetables, fruits and seeds. Tomatoes are thought to be one of the natural media of bacteria because tomatoes contain various chemical compositions that are needed as a substrate for LAB. The purpose of this study is to isolate and determine the type of LAB produced microscopically. The ingredients used are tomatoes obtained from Padang Panjang market, with Merck NA media, oxoid MRSa, 0,9% NaCl, and crystal violet paint. Isolation LAB from tomatoes done in two ways, the first with plant tomato tissue directly into the NA medium and the second with fermentation tomato and then plant the tomato into the MRSa medium with streak plate methods. The isolates obtained were identified microscopically using a microscope with gram staining method. From the research that has been done, the following result are obtained : (1) Plant tomatoes tissue direcly into NA medium and gram staining, there were 1 colonies of gram positive bacteria with bacil cell form. (2). Fermentation tomatoes from the results, there were 2 colonies gram positive bacteria with bacil cell form. We can identify this colonies as Lactobacillius bulgaricus.
Highlights
Prebiotics are good coumpounds used in the growth of lactic acid bacteria (LAB) in digestion, prebiotics come from vegetables, fruits and seeds
Tomatoes are thought to be one of the natural media of bacteria because tomatoes contain various chemical compositions that are needed as a substrate for LAB
Isolation LAB from tomatoes done in two ways, the first with plant tomato tissue directly into the NA medium and the second with fermentation tomato and plant the tomato into the MRSa medium with streak plate methods
Summary
Prebiotik merupakan senyawa yang menguntungkan manusia dengan menstimulasi pertumbuhan dan aktifitas satu atau sejumlah kecil bakteri di koloni. Prebiotik merupakan senyawa yang baik digunakan dalam pertumbuhan bakteri asam laktat dalam pencernaan, prebiotik berasal dari sayur-sayuran, buah-buahan dan biji-bijian. Zat terlarut air yang sebagian besar merupakan fruktosa dan glukosa, serta asam utama yang terkandung didalam tomat adalah asam sitrat. Tomat juga telah terbukti sebagai media pertumbuhan yang baik bagi beberapa jenis bakteri seperti Lactobacillus acidophilus, L casei, L plantarum dan L brevis. Tomat juga mengandung senyawa antioksidan likopen, provitamin A, Vitamin E, vitamin C senyawa fenolik, serta flavonoid yang sangat baik dalam mengurangi resiko timbulnya berbagai macam kanker karena bersifat antioksidan. Peranan utama bakteri asam laktat adalah sebagai kultur starter produkproduk yang melibatkan proses fermentasi untuk memperoleh produk akhir dengan konsistensi tinggi, menstabilkan produk-produk sehingga diperoleh cita rasa yang spesifik serta untuk mengawetkan produk yang diinginkan. Untuk itu perlu dilakukan penelitian yang bertujuan untuk mengisolasi dan menentukan bakteri asam laktat yang ada pada buah tomat
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