Abstract
Lactic acid bacteria (LAB) are ubiquitous and are one of the major microbial groups involved in the fermentation of various types of food. They are the most dominant microbes present in milk or milk products and fermented foods where they play vital roles in both the manufacturing and ripening processes. Kaladhi is one of the traditional fermented products of the North-West Himalaya region. It is a hard and dry cheese. In our research, a total of 9 isolates was isolated and was evaluated on the basis of preliminary characterization viz. morphological as well as biochemical characterization and was examined for their antagonistic activity against following pathogens. On the basis of their maximum antagonistic potential against food-borne pathogenic bacteria, isolate K1 is characterized by 16S rRNA sequencing. The isolate was identified as Lactobacillus paracasei subsp. Tolerans strain NBRC 15906 K1|MN814072|.This research was aimed to study the unexplored microflora of Kaladhi and to determine its probiotic potential.
Highlights
Microorganisms are present almost in every habitat because of their ubiquitous property
Lactic acid bacteria (LAB) are ubiquitous and are one of the major microbial groups involved in the fermentation of various types of food
A total of 9 isolates were isolated from the sample Kaladhi and were recognized on the basis of morphological characteristics and biochemical analysis
Summary
Microorganisms are present almost in every habitat because of their ubiquitous property. Some microbes exhibit various beneficial properties viz. Probiotics are one of those microbes that have an extensive role in maintaining the host’s health and controlling some diseases. According to FAO/ WHO, probiotics are: “Live microorganisms which when administered in adequate amounts confer a health benefit on the host”. They are considered as “friendly bacteria” or “good bacteria”. Probiotic bacteria are non-pathogenic and non-toxic microbes and show important functions for well being and health of the host[1]. It’s important to maintain the ecological flora in order to prevent diseases, especially infections of the gastrointestinal tract[2,3]
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