Isolation and Characterization of Hydrocarbon-degrading Bacteria in Soils of Mechanical Workshops in Maiduguri, Borno State

Haruna Yahya Ismail,Hussaini Shettima,Ibrahim Alkali Allamin,Nasir Halima,Yusuf Musa

doi:10.54987/jebat.v4i2.632

Haruna Yahya Ismail, Hussaini Shettima + Show 3 more

Open Access

https://doi.org/10.54987/jebat.v4i2.632

Copy DOI

Abstract

The study was conducted to determine the distribution of hydrocarbon utilizing bacteria in spent engine oil (SEO) contaminated soil. Five mechanical workshops within Maiduguri Metropolis. Five bulk soil sample comprising of one each of the five sites; the sites are Leventis Area on Kashim Ibrahim Way, Damboa Raod, Boiler (commonly called Bola) near Maiduguri Monday Market, Ngomari, on Kano Road, and 1000 Housing Estate, also on Kano Road. Nutrient agar was used, or isolation and enumeration total heterotrophic bacteria and Mineral salt agar was used for the isolation and enumeration of hydrocarbon utilizing bacteria. The result shows THB (92.0x104) at Damboa road (DR) to as higher and (78.8x104) at Bola area (BL) while HUB (2.0x104) at Damboa road (DR) and as high as (9.3x104) at Bola area (BL). The bacterial species isolated were species of Bacillus licheniformis, Bacillus subtilis, Bacillus coagulans, Bacillus alvei, Bacillus cereus, Bacillus lentus other are Pseudomonas aeruginosa, Klebsiella pneumonia. Bacillus licheniformis, and Bacillus subtilis are the most occurring bacterial isolates identified. The ability of those bacterial isolate to degrade hydrocarbon buoyantly will help in remediation of oil polluted environments.

Highlights

Human activity has had an increasing impact on many ecosystems in the last several years, as seen by the numerous changes that have occurred
The study sites are five different mechanical workshops in Maidudguri metropolis located at Bola Workshop near the Post Office, Anthony Mechanical workshop along Dambo Road, Leventis Mechanical Workshop Near West-End, Ngomari Workshop near Maiduguri International Airport, and 1000 Housing Estate along Kano Raod
Isolation and enumeration of Bacteria Bacterial load of the soil samples were enumerated by making tenfold dilution of the samples collected from the soil samples

Summary

Introduction

Human activity has had an increasing impact on many ecosystems in the last several years, as seen by the numerous changes that have occurred. We are not aware of any study that has attempted to isolate and identify bacteria present in the used motor oil polluted soil environment in Maiduguri.

Results

Conclusion