Abstract
Objective: Mesenchymal stromal cells (MSCs) are considered as an excellent source in regenerative medicine, but availability and ethical problems limited their routine use. Therefore, another available source with easy procedure and exempt from ethical debate is important. The purpose of this study is to isolate and characterize the MSCs from human placenta. The stromal cells were isolated from Placental Decidua Basalis (PDB-MSC), Umbilical cord Wharton’s Jelly (WJ-MSC) and Amniotic Membrane (AM-MSC). Methods: Full term human placentas (n=4), from cesarean section delivery were collected. Small fragments from different parts were cultures as explants. The immunophenotyping, mesodermal differentiation, growth kinetics and stemness gene expression was studied. Results: The cultivated cells from three sources expressed CD44, CD105, and CD90. Gene expression of NANOG and OCT4 confirmed the undifferentiated state. The doubling-times for WJ-MSCs, PLC-MSCs and AM-MSCs, respectively, were 21±8h, 28±9h and 25±9h at passage three and 30±5h, 45±7h and 45±7h at passage tenth. The proliferative potential of WJ-MSCs tended to be higher than the other two sources. Conclusion: The fetal derives stromal cells; especially the early passages of WJ-MSCs are available supplies for large scale production of MSC for using in clinical studies or research projects.
Highlights
Mesenchymal stromal cell (MSC) is defined by adherency to plastic tissue culture plates and capacity to differentiate into mesenchymal lineages with certain cell surface markers
The initial growth of AM-MSC at primary culture (P0) consisted of adherent cells with heterogenous population; one of them was spindle shaped fibroblast-like cells and another one was epithelial-like morphology
The MSCs were expanded till passage 10 (P10)
Summary
Mesenchymal stromal cell (MSC) is defined by adherency to plastic tissue culture plates and capacity to differentiate into mesenchymal lineages with certain cell surface markers. MSCs are cells with high in vitro self renewal capacity and ability to differentiate into multiple mesoderm, ectoderm and endoderm lineages. They possess antiinflammatory and immuno-modulatory effects and are considered as promising tool for cell based therapeutic strategies[1,2,3] such as induction of tolerogenic response in graft vs host disease GVHD2,3 and enhanced antitumor therapy.[4,5]. As a medical waste, is usually discarded without any ethical conflict.[8] This fetal tissue is considered as a good available source of MSCs for stem cell therapy. The human placenta is composed of amnion, chorion and deciduas.[8]
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