Isolation and characterization of human DNA recovered from Cimex hemipterus (F.) (Hemiptera:Cimicidae).

Abstract

The ability to isolate and generate a DNA profile from human DNA recovered from tropical bed bugs (Cimex hemipterus) for identifying individuals can be useful for public health, forensic, and medical entomology. In this study, genomic DNA was recovered from both male and female bed bugs at every time interval tested (0, 1, 3, 5, 7, 14, 30, and 45days post blood meal). The total DNA concentrations recovered from male bed bugs ranged from 12.93 to 65.97ng/µL, while the total DNA concentrations from female bed bugs ranged from 8.93 to 44.53ng/µL. However, based on the results from the BLAST search and PCR products, human DNA could be detected from female bed bugs at 0, 3, 5, 14, and 30days post blood meal using the D18S51 marker. Concentrations of PCR products of the D18S51 locus from male bed bugs ranged from 4.20 to 35.50ng/µL, whereas, for female bed bugs, concentrations ranged from 4.31 to 22.47ng/µL. These were generally higher compared to the PCR products of the first hypervariable part (HVR1) marker. The results indicate the HVR1 locus was less sensitive than the D18S51 locus.