Abstract

Growth hormone (GH) was purified from pituitary glands of tuna (Thunnus albacares). The yield of this hormone was 4mg/g wet tissue. The hormone had a molecular weight of 21,000 and an isoelectric point of 7.1. The partial amino acid sequence including N-terminal Gin, which was modified to pyroglutamate, was established by analyzing peptide fragments generated by chemical and enzymatic treatments. Intraperitoneal injection of tuna GH at doses of 0.1 and 1 μ body weight at 7 day intervals resulted in a significant increase in body weight and length of juvenile rainbow trout (Salmo gairdneri). The GH-treated fish had a 3-fold higher growth rate and a 1.6-fold higher food conversion efficiency than the control fish.

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