Abstract
We have previously reported the presence of GM2 as the major ganglioside in the roe of striped mullet, Mugil cephalus, (Li, Y.-T., Hirabayashi, Y., DeGasperi, R., Yu, R. K., Ariga, T., Koerner, T. A. W., and Li, S.-C. (1984) J. Biol. Chem. 259, 8980-8985). In addition to GM2, mullet roe also contain a series of gangliosides with thin-layer chromatographic mobilities slower than GM2. Besides enzymatic hydrolysis and NMR spectroscopy, we have employed the thin-layer chromatography overlay technique using a human monoclonal IgM antibody which recognizes the GM2 epitope to study the nature of these gangliosides. Using these methods we have isolated and characterized three novel mullet roe gangliosides with the following structures: (Formula: see text). These three gangliosides all contain neolacto-series sugar chains. However, the unique feature of gangliosides 5 and 10 is that the terminal portion of the sugar chain is of the ganglio-series while the internal portion is of the neolacto series structure. Due to the substitution of a GalNAc on the internal Gal in 9 and 10 in the inner core, these two gangliosides also contain the gangliotriaosyl structure. Thus, the sugar chains in these gangliosides are of novel type and can be considered a hybrid between the two series which can be defined as the neolacto-ganglio series.
Highlights
We have previously reported thepresence of GM2as the major ganglioside in theroe of striped mullet, Mug.1 cephalzm
In addition to GM2, mullet roe contain a series of gangliosides with thin-layer chromatographicmobilition of a GalNAc on the internalGal in 9 and 10 in the innercore, these two gangliosides contain the gangliotriaosyl structure
The sugar chains in these gangliosides are of novel type andcan be considered a hybrid between the two series which can be defined as the neolacto-ganglio series
Summary
National Institutes of Health and DMB 86-17033from the National est grade were obtained from commercial sources. Susceptible to both human hepatic ,&hexosaminidase A and Glycosiduse Treatments-Treatments of gangliosides with endo-p- endo-6-galactosidase, we concluded that these gangliosides galactosidase, Cer-glycanase, and neuraminidase, respectively, were performed as described previously (5, 7, 16). After washing with the same solventto remove the unad- an additional ganglioside with TLC mobility close to that of sorbed material, the column was eluted with 800 ml of chloroform/ methanol (2:7) containing 10 mM sodium acetate. 140-150 were pooled and refractionated by a Bio-Si1A column to remove 3.Fractions [160-170] contained a ganglioside with TLC mobility slower than thatof ganglioside fraction A. This ganglioside was initially called ganglioside fraction B. One-dimensional proton nuclear magnetic resonance spectra were obtained ona Bruker WM 500 Spectrometer a t 30 "C
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