Isolation and Characterization of fim-C S. Typhi Gene 0.8 Kilo Base as a Preliminary Study to Discover a Candidate of Recombinant Vaccine for Typhoid Fever

Abstract

Salmonella typhi is bacteria that cause typhoid fever for human. In Indonesia, the morbidity rate of typhoid fever’s suffer tends to increase. The common method to prevent typhoid fever is to weaken the vaccine of S. typhi bacteria. However this kind of vaccine has weaknesses that occur in several side effects, such as nausea, vomit, fever, and headache. Therefore this research is aimed to isolate and to characterize the gene structure of fim-C S. Typhi local strain of 0.8 kilobase as an initial study to develop the recombinant vaccine. This research used explorative method that consists of (1) designing the specific primer of fim-C S. typhi gene; (2) synthesize the specific primer pair of fim-C S. typhi gene; (3) isolate the genome of S. typhi bacteria; (4) amplificate the fim-C gene by PCR; (5) characterize the amplification result fim-C S. typhi gene. The specific primer from the designing process (Fw 1b-Rev 1a New) has succeeded to amplify the S. typhi genome showed by one band of 0.8 kilobase by using gel agarose electrophoresis. The characterization of 0.8 kilobase band from the amplification result by using sequencing technique succeded to detect 766 base pairs. It is concluded that this research has succeeded to isolate and to characterize fim-C gene S. typhi local strain of of 0.8 kilobase. This fim-C gene has possibility for developing a recombinant vaccine candidate in the further study.