Abstract
In the present study, expressed sequence tag-based microsatellite markers were developed by 454 pyrosequencing for the marine fish Scatophagus argus. A total of 510,939 reads were obtained, and 4,322 di-nucleotides, 1,354 tri-nucleotides, 219 tetra-nucleotides, 30 penta-nucleotides and 9 hexa- nucleotides were detected. Among these repeated sequences, 51 microsatellites where repeat units were greater than 4 were randomly selected to test the variability in natural populations. 12 polymorphic markers were identified and characterized. The number of alleles per locus varies from 3 to 6, and observed and expected heterozygosities ranged from 0.102 to 0.658 and from 0.124 to 0.691, respectively. No genetic linkage and significant departure from Hardy–Weinberg equilibrium were detected among these loci. High-throughput pyrosequencing allows fast and efficient isolation of microsatellite markers on a large scale, and can provide enough microsatellite markers for population genetic studies and genetic mapping.
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