Abstract
We isolated and characterized eleven polymorphic tetranucleotide microsatellite loci in the spiny-bellied frog (Quasipaa boulengeri). The loci were screened in 62 individuals from two natural populations. All loci were variable, with the number of alleles ranging from 8 to 26. The average observed and expected heterozygosity per locus ranged from 0.500 to 0.947 and from 0.735 to 0.932, respectively. Two loci were found to be significant departure from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was detected after sequential Bonferroni corrections. These polymorphic loci will be useful for conservation and population genetics studies of this threated species.
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