Abstract
Poecilus cupreus (L.) (Coleoptera: Carabidae) is a typical polyphagous predator species on arable lands. In this study, 11 polymorphic microsatellite markers were developed from genomic DNA of P. cupreus. Genotypes of 87 individuals sampled from three localities in Western France were analyzed to characterize the polymorphism at each locus. The number of alleles ranged from 3 to 20. All pairs of loci were in linkage equilibrium. The expected and observed heterozygosity ranged from 0.165 to 0.890 and 0.103 to 0.926, respectively. From the northern to the southern sites, six, three, and six loci showed significant deviations from Hardy-Weinberg equilibrium, respectively. At one locus, null alleles were suspected as occuring at high frequency in the three populations. A weak ge- netic differentiation among populations was found, suggesting intense and large scale gene flow coupled with large population size in P. cupreus. These polymorphic microsatellite markers could be used in future population genetics studies of P. cupreus.
Highlights
Carabid beetles living within agroecosystems are considered as potential natural enemies of many crop pests and may provide important and valuable ecosystem services as predators of a range of arthropods, for example, aphids (Losey & Vaughan, 2006)
The present study reports on 11 polymorphic microsatellite markers that may potentially be useful in future investigations of these aspects in this particular important predatory insect
Eleven polymorphic microsatellite markers were developed for the carabid beetle, P. cupreus
Summary
Carabid beetles living within agroecosystems are considered as potential natural enemies of many crop pests and may provide important and valuable ecosystem services as predators of a range of arthropods, for example, aphids (Losey & Vaughan, 2006). The mosaic of habitat patches constituting agricultural landscapes and the crop management practices affect carabid beetle abundance and population dynamics (Holland & Luff, 2000). This spatio-temporal heterogeneity of agricultural landscapes makes dispersal crucial for long-term survival of carabid species and for their effectiveness as natural enemies of crop pests. The ground beetle, Poecilus cupreus (Linnaeus, 1758) (Coleoptera: Carabidae) is a typical polyphagous predator species commonly found inhabiting arable-ecosystems throughout Europe (Jeannel, 1942; Langmaack et al, 2001) It has developed wings (Bommarco, 1998; Hendrickx et al, 2009) but it is more likely to disperse mainly by walking (Wallin, 1985; Hendrickx et al, 2009); it is able to run up to 10.6 cm.s–1 at 20°C (Thiele, 1977). The present study reports on 11 polymorphic microsatellite markers that may potentially be useful in future investigations of these aspects in this particular important predatory insect
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