Abstract
Dendrobium huoshanense (Orchidaceae) is a perennial herb and a widely used medicinal plant in Traditional Chinese medicine (TCM) endemic to Huoshan County town in Anhui province in Southeast China. A microsatellite-enriched genomic DNA library of D. huoshanense was developed and screened to identify marker loci. Eleven polymorphic loci were isolated and analyzed by screening 25 individuals collected from a natural population. The number of alleles per locus ranged from 2 to 5. The observed and expected heterozygosities ranged from 0.227 to 0.818 and from 0.317 to 0.757, respectively. Two loci showed significant deviations from Hardy-Weinberg equilibrium and four of the pairwise comparisons of loci revealed linkage disequilibrium (p < 0.05). These microsatellite loci were cross-amplified for five congeneric species and seven loci can be amplified in all species. These simple sequence repeats (SSR) markers are useful in genetic studies of D. huoshanense and other related species and in conservation decision-making.
Highlights
Dendrobium huoshanense, a perennial epiphytic orchid herb, is endemic to Huoshan County town in Anhui province in southeast China
Deviations from Hardy-Weinberg expectation (HWE) were detected for each locus, nine loci conformed to HWE and two loci (Hs39, Hs50) significantly deviated from HWE in the sampled population after Bonferroni’s correction (p < 0.05)
Seven loci (Hs39, Hs41, Hs44, Hs47, Hs48, Hs56, Hs57) can be amplified in all five species, while three loci (Hs40, Hs50, Hs54) can be amplified in four species and one loci (Hs42) can be amplified in two species. These results suggest that the identified microsatellite markers are useful in genetic studies of D. huoshanense and other Dendrobium species
Summary
Dendrobium huoshanense, a perennial epiphytic orchid herb, is endemic to Huoshan County town in Anhui province in southeast China. SSRs (simple sequence repeats), known as microsatellites, have become the most popular source of genetic markers owing to their high reproducibility, multi-allelic nature, codominant inheritance, high abundance, and wide genome coverage [5,6]. They are an ideal tool for genome mapping, population genetics, conservation biology, marker-assisted selection and other studies [7,8,9,10]. We report the isolation and characterization of eleven polymorphic microsatellite loci from D. huoshanense using a modified biotin-capture method for the first time [13]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
