Isolation and characterization of cyclic α-(1→4)-glucans having degrees of polymerization 9–31 and their quantitative analysis by high-performance anion-exchange chromatography with pulsed amperometric detection

Abstract

Cyclic α-(1→4)-glucans with degrees of polymerization (DPs) 9–31 were isolated from a mixture of cyclization products formed in the early stage of the action of cyclodextrin glucanotransferase (CGTase) on synthetic amylose, and characterized by matrix-assisted laser desorption ionization time-of-flight MS, 13C-NMR and HPLC of their partial acid hydrolyzates. High-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection for an accurate estimation of cyclic α-(1→4)-glucans was developed using those isolate glucans as quantification standards, and by HPAEC, the time course of the cyclization reaction of CGTase from an alkalophilic Bacillus sp. A2-5a on synthetic amylose was determined.