Abstract
The objective of this research was to isolate and characterize collagenase of Bacillus thuringiensis obtained from the collection of Aquatic Biotechnology Laboratory, Faculty of Fisheries and Marine Science, Padjadjaran University. The substrate of collagen was derived from skin Tilapia waste of Cirata Reservoar. This study showed the presence of clear zone which is a sign of colagenolitik activity of B. thuringiensis. The optimum production time of collagenase was 24 hours of incubation. Collagenase of crude extract had collagenase activity of 0.181 units/ml with the protein concentration was 0.640 mg/ml. It was also found that the optimum temperature of collagenase derived from crude extract was 50° C and the optimum pH was 7-9. Keywords: collagenase; Bacillus thuringiensis; skin, waste
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
