Abstract

A CO 2-fixing bacterium, strain YN-1, that can fix CO 2 under chemoautotrophic conditions but not photoautotrophic conditions was isolated from seawater. Identification of the isolate was carried out using biochemical tests and 16S rDNA sequence analysis, and its characteristics were investigated. From the results of partial 16S rDNA sequence analysis, strain YN-1 showed low identity with previously reported hydrogen-oxidizing bacteria, Hydrogenovibrio marinus MH-110 and Hydrogenophilus thermoluteolus. This result indicates that strain YN-1 may be a new hydrogen-oxidizing marine bacterium. Strain YN-1 showed considerable CO 2 fixation ability during continuous cultivation even at high CO 2 concentration. Strain YN-1 used H 2 and CO 2 as energy and carbon sources, respectively. Growth characteristics were examined in batch and continuous cultivation with a view to improving the CO 2 fixation rate. The results showed that CO 2 fixation occurred in the absence of a light source and that the strain exhibited good growth at high CO 2 concentration (40%). On the other hand, the dry cell weight was 13.4 g/ l following continuous cultivation for 76 h in 10% CO 2 (0.1 l/min), and at that time the amount of fixed CO 2 was 18.08 g CO 2/ l. This indicates that strain YN-1 can efficiently fix CO 2 even at high CO 2 concentrations, which would allow its application to the removal of industrially discharged CO 2.

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