Abstract
A partial gene library of Pseudomonas syringae pv. pisi race 2 strain 203 was constructed in the broad host range cosmid pLAFR3. Individual clones from this library were transferred by a replica plate mating procedure to P. syringae pv. pisi race 1 strain PF247. Mobilization of the clones from Escherichia coli involved triparental mating with a conjugative helper plasmid. Transconjugants bearing the library clones were stem-inoculated into pea ( Pisum sativum) cultivar Early Onward (susceptible to race 1, but resistant to race 2). A single clone, designated pAV270, conferred race-specific avirulence towards a single dominant host gene R2, segregating in an F 2 population of the crosses Kelvedon Wonder (susceptible to both race 1 and 2) × Early Onward and Kelvedon Wonder × Fortune (also carrying R2 in addition to other resistance genes). Subcloning of pAV270 in pLAFR3 indicated that the gene (designated avrAspi1) responsible for the observed phenotype was located on a 4·0 kb EcoRI fragment of strain 203 DNA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
