Abstract
A partial gene library of Pseudomonas syringae pv. pisi race 2 strain 203 was constructed in the broad host range cosmid pLAFR3. Individual clones from this library were transferred by a replica plate mating procedure to P. syringae pv. pisi race 1 strain PF247. Mobilization of the clones from Escherichia coli involved triparental mating with a conjugative helper plasmid. Transconjugants bearing the library clones were stem-inoculated into pea ( Pisum sativum) cultivar Early Onward (susceptible to race 1, but resistant to race 2). A single clone, designated pAV270, conferred race-specific avirulence towards a single dominant host gene R2, segregating in an F 2 population of the crosses Kelvedon Wonder (susceptible to both race 1 and 2) × Early Onward and Kelvedon Wonder × Fortune (also carrying R2 in addition to other resistance genes). Subcloning of pAV270 in pLAFR3 indicated that the gene (designated avrAspi1) responsible for the observed phenotype was located on a 4·0 kb EcoRI fragment of strain 203 DNA.
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