Isolation and characterization of cerebral microvascular pericytes.

Abstract

AbstractAlthough a plethora of information exists on the role of the endothelial cell (EC) in vascular hemostasis and tissue homeostasis, little is known of the role played by the microvascular pericyte (PC) (Fig. 1). This lack of substantial information is most evident in the understanding of the role played by the PC in blood-brain barrier (BBB) function, and in the pathophysiology of central nervous system (CNS) disease. Development of techniques for the isolation of defined populations of CNS microvessels (1–3), for the preparation of retinal PC (3,4), and for the preparation of cerebrovascular PC (5–7) have enabled scientists to examine the function of this unique cell in the brain (8). The subculture of PC from purified preparations of cerebral microvessels will be discussed below. All populations, isolated microvessels, enriched PC and EC cultures, pure primary cultures, and experimentally derived co-cultures, are suitable in experimental protocols modeling BBB function.CNS pericytes were isolated from cerebrovascular microvessels. One-week-old cultures display typical spreading with irregular projections as shown on light bright-field microscopy. ×100 KeywordsEndothelial Cell CultureTeflon PestleConical Centrifuge TubeMicrovascular PericyteMicrovessel FragmentThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.