Abstract
1. 1. Brush border membrane vesicles (BBMV) were isolated from swine mid-intestine by a MgCl 2 precipitation and sucrose density gradient centrifugation. 2. 2. Transport of d-glucose and l-alanine were Na +-stimulated and into an osmotically sensitive space. 3. 3. Estimates of kinetic parameters for Na +-dependent d-glucose transport were: apparent K t = 1.8 mM and J max = 16.8 nmol/mg protein/min. 4. 4. Results of experiments with the ΔpH sensitive fluorescent probe 9-aminoacricline indicated indepen- dent mechanisms for Na +-dependent glucose transport and Na +/H + exchange. 5. 5. This study demonstrates that pig BBMV provide a useful model for investigating intestinal membrane transport.
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