Abstract
1. 1. Boar semen was separated on a Percoll density gradient into three populations; a low-density band of immature sperm cells containing a cytoplasmic droplet and a high-density doublet band formed by spermatozoa without a cytoplasmic droplet. 2. 2. In these three cell populations four acid hydrolases were determined, viz. (1) α-glucosidase; (2) α-mannosidase; (3) β-galactosidase; (4) β-hexosaminidase. 3. 3. The release of the hydrolases (1), (2) and (3) from cytoplasmic droplet containing spermatozoa was stimulated whereas the release of β-hexosaminidase was inhibited by calcium ions. 4. 4. The results suggest that acid α-glucosidase, α-mannosidase and β-galactosidase are situated in the acrosome whereas acid β-hexosaminidase is localized predominantly in the cytoplasmic droplet of boar spermatozoa. 5. 5. We conclude that β-hexosaminidase should prove useful as a biochemical marker for cytoplasmic droplet containing spermatozoa and hence for the number of immature sperm cells in boar semen.
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