Abstract
This study aimed to isolate and identify biosurfactant producing and diesel alkanes degrading bacteria. For this reason, bacteria isolated from the diesel contaminated site were screened for their potential to produce biosurfactants and degrade diesel alkanes. Primary selection of diesel degraders was carried out by using conventional enrichment culture technique where 12 bacterial strains were isolated based on their ability to grow on minimal media supplemented with diesel as sole carbon source, which was followed by qualitative screening methods for potential biosurfactant production. Isolate B11 was the only candidate that shows positive signs for drop collapse, foaming, haemolytic test, oil displacement of more than 22 ± 0.05 mm, and emulsification (E24) of 14 ± 0.30%. The effect of various culture parameters (incubation time, diesel concentration, nitrogen source, pH and temperature) on biodegradation of diesel was evaluated. The optimum incubation time was confirmed to be 120 days for isolates B11, the optimum PH was confirmed as 8.0 for the isolate, Similarly, the optimum temperature was confirmed as 35oC. In addition, diesel oil was used as the sole carbon source for the isolates. The favourable diesel concentration was 12.5 % (v/v) for the isolate. The isolate has shown degradative ability towards Tridecane (C13), dodecane, 2, 6, 10-trimethyl- (C15), Tetradecane (C14), 2,6,10-Trimethyltridecane (C16), Pentadecane (C15). It degraded between 0.27% - 9.65% individual diesel oil alkanes. The strain has exhibited the potential of degrading diesel oil n-alkanes and was identified as Alcaligenes species strain B11 (MZ027604) using the 16S rRNA sequencing.
Highlights
Petroleum hydrocarbons are a major environmental contaminant that harms the surrounding ecosystem [1]
This study looked at the isolation and identification of biosurfactant-producing bacteria to see if they might degrade diesel n-alkanes
The results revealed that the isolate B11 grew better on mineral salt medium supplemented with Urea than on NH4NO3 (Fig. 5)
Summary
Petroleum hydrocarbons are a major environmental contaminant that harms the surrounding ecosystem [1]. Because of the toxicity and carcinogenicity of petroleum products, the accidental release of hydrocarbons into soils due to transportation, spillage, and storage tanks is a major environmental concern [3]. Soil pollution caused by petroleum hydrocarbon contamination is a serious global issue. Given the amount of oil used and transported around the world, the extent of hydrocarbon contamination in the environment is unsurprising [4]. The bioremediation process has been found to be an effective method for hydrocarbon remediation [5]. Biodegradation of hydrocarbon-contaminated soils, which takes advantage of microorganisms' ability to degrade organic contaminants, has been established as an efficient, cost-effective, and environmentally friendly method [6]. The purpose of this research is to isolate and identify biosurfactant producing bacteria as well as their diesel oil degradation potential
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
