ISOLATION AND CHARACTERIZATION OF BIOLOGICALLY ACTIVE SECONDARY METABOLITES FROM METASEQUOIA GLYPTOSTROBOIDES MIKI EX HU

Abstract

ABSTRACTAn increase in foodborne diseases has prompted the search for novel antibacterial agents to control foodborne pathogens. In this study, an abietane type diterpenoid, sugiol was isolated from Metasequoia glyptostroboides using a column chromatographic analysis. The structure of the compound was elucidated by spectroscopic data analysis. The sugiol (50 µg/disc) showed potential antibacterial effect as diameters of inhibition zones (10.2 ± 0.3 to 16 ± 0.4 mm) against foodborne pathogenic bacteria. The minimum inhibitory and minimum bactericidal concentrations values of sugiol against the employed bacteria were found in the range of 62.5 to 250 and 125 to 250 µg/mL, respectively. Also the compound had strong antibacterial effect on the viable counts of the tested bacteria. Scanning electron microscopic study demonstrated potential detrimental effect of sugiol on the morphology of Listeria monocytogenes ATCC 19166. These findings indicate that sugiol could be a possible candidate for using in food industry to control foodborne pathogens.PRACTICAL APPLICATIONSFoodborne pathogens are the leading causes of illness and death in developed countries. These pathogens are responsible for millions of cases of infectious gastrointestinal diseases each year, costing billions of dollars in medical care and lost productivity. New foodborne pathogens and foodborne diseases are likely to emerge driven by factors such as pathogen evolution, changes in food manufacturing practices, and changes to the human host status. Fuelled by these concerns, research on plant‐based natural and biologically active secondary metabolites to control the foodborne pathogens has escalated to unprecedented levels in recent years. Plant‐based antimicrobials have been found to suppress the growth of foodborne pathogens in various in vitro and in vivo models. The findings of this study clearly indicate that biologically active diterpenoid, sugiol isolated form Metasequoia glyptostroboides could be used as an affective candidate to control the growth of certain important foodborne pathogens.