Abstract
An extracellular acidic polysaccharide produced by Serratia piscatorum IFO 12527 was found to exhibit a marked antiinflammatory activity. The polysaccharide was purified by fractional precipitation with cetyltrimethylammonium bromide and then by gel filtration on Sepharose 2B to give two homogeneous fractions, PLS N–I and PLS N–II, the former exhibiting the antiinflammatory activity.PLS N-I was a complex polysaccharide composed of l-rhamnose, d-galactose and d-galacturonic acid in the molar ratio of 2: 1; 1, together with small portions of d-glucosamine, d-galactosamine, protein and fatty acids such as acetic, lauric, myristic, β-hydroxyrnyristic and palmitic acids. Physicochemical and biological properties of PLS N–I and PLS N–II were also described.
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