Isolation and Characterization of Bacterial Lipids and its Application as Antioxidant Agent

Abstract

The study was aimed to isolate the oleaginous bacteria from various soil samples and characterize the bacterial lipids to study its suitable application. The study was carried out from June 2023 to October 2023 in the microbiology lab at Changu Kana Thakur Arts, Commerce & Science College, New Panvel (Autonomous). The soil samples were collected from the garden, dumping, and compost area at New Panvel. The bacterial isolation was carried out by simple streak plate method, followed by screening by Sudan black B staining method. Production of oleaginous bacteria was done, followed by extraction of lipids by Bligh and dyer method. The extracted lipids were characterized by TLC and FTIR. The antioxidant potential of extracted lipids was assessed by DPPH method. The different oleaginous bacteria were isolated from soil sample and screened by Sudan black B staining method. The results indicate phospholipids, TAG, esters of fatty acids were mainly present. The extracted lipid samples S1C2, S2C4, S2C2, S1C1, S3C4, S2C1, S3C2 have 83.30%, 80%, 36.60%, 23.30%, 26.60%, 16.6%, 6.6% antioxidant potential respectively.