Abstract

Eighteen bacterial strains were isolated and characterized from three ornamental plant species growing in vitro. The highest incidence of contamination in the explants was found in Dieffenbachia (80%), followed by Anthurium (65%), while only 30% of Spathiphyllum explants were contaminated. Of the isolated microorganisms, 73% were Gram-negative and 27% Gram-positive. The methods used to characterize the isolates were sufficient to identify their genus. Two independent identification criteria were used and most of the 18 isolates showed similarity between the criteria. When the purified bacterial isolates were exposed to antibiotics, each isolate was sensitive to several of the agents tested, but only trimethoprim-sulfametoxazol was effective as a bactericide for all the isolates.

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