Isolation and characterization of Azotobacter sp. for plant growth promotion and abiotic stress tolerance in Telangana's agro-climatic regions: Towards sustainable development

Abstract

Current study was done to understand and improve plant survival and growth under biotic and abiotic stress tolerance was considered. The primary goal of this work was to isolate azotobacterial species from indigenous places where the agriculture community has been practicing traditional methods along with regular places, also examined the areas of stressed agriculture areas which are seasonally cultivated and environments in their capacity to enhance drought stress tolerance brought from different crop fields such as Maize, sweet Sorghum, Sugarcane, corn, and wheat to increase the yield.These crops soil samples were taken from few drought areas tolerant places, i.e. under different temperature, salinity, pH and moisture conditions of those particular areas in Telangana. The different soil samples isolated for azotobacter strains from different regions in Telangana were collected and analyzed for growth in media to get the high yield for the agriculture purpose which will benefit the agricultural community and leads to sustainable development. Morphological, biochemical, and molecular characteristics were used to evaluate the Azotobacter species. Azotobacter beijerinckii strain and Azotobacter tropicalis strain were identified by biochemical testing and 16s rRNA sequencing. Azotobacter beijerinckii strain (BKPOU06TS) and Azotobacter tropicalis strain (BKPOU08TS) 16S rRNA sequences were submitted to GenBank with accession numbers OP536202 and OP536206, respectively.