Isolation and characterization of Antarctic psychrotrophStreptomycessp. strain INACH3013

Abstract

AbstractAn actinobacterial strain with antimicrobial activity, INACH3013, was isolated from soil collected from Antarctica. The taxonomic status of the isolate was established using a polyphasic approach. The strain was identified as belonging to the genusStreptomycesbased on the scanning electron microscopic observation and partial 16S rRNA gene sequence analysis. The sequence analysis revealed that strain INACH3013 is closely related toStreptomyces fildesensis(99.8%),S. beijiangensis(98.1%) andS. purpureus(97.2%). A phylogenetic tree constructed using the partial 16S rRNA gene sequences of strain INACH3013 and closely related strains revealed that INACH3013 fell into the same subclade asS. fildesensisandS. purpureus. Strain INACH3013 was observed to be psychrotolerant, slightly halotolerant (up to 5% NaCl) and capable of inhibiting the growth of seven Gram-negative and eight Gram-positive foodborne pathogens. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of the strain is KJ624755.