Abstract
Soil sample was collected from the coastal region of Tamil Nadu with the aim of isolating actinobacteria and screen them for antagonistic activity against common bacterial and fungal pathogens. Serial dilution of the soil sample and subsequent screening of the isolates obtained, resulted in the identification of a potential strain VITDDK2 with significant activity against Klebsiella pneumoniae, Aspergillus flavus and Aspergillus niger. In addition, the strain VITDDK2 also possessed chitinolytic activity. Chemotaxonomic analysis showed that the isolate VITDDK2 belongs to cell wall Type I. 16 S rRNA partial gene sequence and phylogenetic analysis showed that the strain VITDDK2 shared 93% similarity with Streptomyces sp. strain 346. Also the secondary structure of the rRNA of VITDDK2 and the restriction sites were predicted using Genebee and NEBCutter softwares respectively.
Highlights
In this long run to combat drug resistant microorganisms, the unexplored and less explored marine environments needs to be studied frequently
In the present study we have reported the isolation and identification of a marine actinobacteria from Ennore saltpan with moderate antibacterial, antifungal and chitinolytic activity
An actinomycete having antagonistic activity was isolated from the soil sample and characterized phylogenetically
Summary
Division of Biomolecules and Genetics, School of Biosciences and Technology, VIT University, Vellore- 632014, Tamil Nadu, India. Soil sample was collected from the coastal region of Tamil Nadu with the aim of isolating actinobacteria and screen them for antagonistic activity against common bacterial and fungal pathogens. Serial dilution of the soil sample and subsequent screening of the isolates obtained, resulted in the identification of a potential strain VITDDK2 with significant activity against Klebsiella pneumoniae, Aspergillus flavus and Aspergillus niger. Chemotaxonomic analysis showed that the isolate VITDDK2 belongs to cell wall Type I. 16 S rRNA partial gene sequence and phylogenetic analysis showed that the strain VITDDK2 shared 93% similarity with Streptomyces sp. The secondary structure of the rRNA of VITDDK2 and the restriction sites were predicted using Genebee and NEBCutter softwares respectively
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
