Abstract
A PLA 2-inhibitor has been purified from Indian cobra ( Naja naja naja) venom by the combination of ion-exchange and gel-filtration chromatography. The inhibitor, NN-I 3 was a peptide with mol.wt 6500 and has a fluorescence emission maxima ca 340 nm. NN-I 3 specifically inhibited the enzyme activity of the three acidic PLA 2 from the same venom. The inhibition of NN-I 2d-PLA 2 and NN-I 2e-PLA 2 by NN-I 3 was of mixed type and NN-I 2c-PLA 2 was of uncompetitive type. Neither the inhibitor nor the individual mixtures of acidic PLA 2 with the inhibitor (1:1 w/w or 1:2 mol:mol) were lethal to mice when injected intraperitoneally in doses upto 10 mg kg −1 body weight.
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