Abstract
A strain of alkaliphilic and halotolerant, rod-shaped bacterium, designated strain SA-3, was solated from an Extreme soda saline-alkali soil in Heilongjiang, northeastern China. Strain SA-3 was Gram-positive, catalase-positive, and oxidase-negative and formed Orange colonies, and starch is not hydrolyzed, optimal growth conditions were pH 9.0 and 0.5 mol/L NaCl. Strain SA-3 has strong resistance to Na 2 CO 3 , hight pH value, and NaCl. 16S rRNA a gene sequence alignment analysis show that the strain formed a separated line within a clade containing the genus Nesterenkonia in the phylum Actinobacteria and was related to the species Nesterenkonia lutea YIM 70081 T (sequence similarity 99.2%), Nesterenkonia sandarakina YIM 70009 T (99.2%), Nesterenkonia jeotgali JG-241 T (99.1%), Nesterenkonia halotolerans YIM 70084 T (99.0%), Nesterenkonia aethiopica DSM 17733 T (97.5%). This similarity, suggest that this salt-tolerant alkaliphilic bacterium belonged to genus Nesterenkonia . This strain is a great potential microbial resources for development.
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