Isolation and Characterization of Acid-Soluble Collagen from the Skin of <i>Amiurus nebulosus</i>

Abstract

During fish processing, a large amount of waste, of the original raw materials is generated, such as skin, bone, scale, viscera and head. These useful resources have been mainly used as feedstuff or fertiliser with low value. To make more effective use of underutilized resources, collagen was isolated from the skin of Amiurus nebulosus using acetic acid and characterized for their potential usage in commercial applications. The yield of acid-soluble collagen (ASC) was 62.05% while the maximum absorbance of ASC was at 234 nm. Amino acid composition and SDS - PAGE suggested that the collagen is possibly possessive of type I collagen. Moreover, FTIR investigations showed the existence of helical arrangements of collagen where the denaturation temperature (Td) and shrinkage temperature (Ts) were 29.8°C and 65.12°C, respectively. There is a possibility that ASC could be utilized as an alternative source of collagen for food, cosmetic, biomedical and pharmaceutical purposes.