Abstract
A yellow pigment was extracted by n-butanol from the sonically disintegrated washed cells of a PABA-requiring strain M48-34 of Escherichia coli following incubation with PABA. These cells were grown in medium containing an inhibitory concentration of sulfathiazole. The pigment contains p-aminobenzoic acid, glutamic acid, alanine, and leucine as components, and has no folic acid activity in microbiological assays. The pigment, used in place of PABA, promoted the growth of strain M48-34. It was found convertible by the washed cells of strain M48-34 to a folic acid-like substance capable of promoting the growth of Streptococcus faecalis R.
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