Abstract
An acidophilic strain was isolated from drainage in Shanxi coal mine, after 16SrDNA amplification and sequencing analysis, it was identified to be Acidithiobacillus ferrooxidans. The ferrous ion oxidation rate of At.f and pH value were studied during its growth, the results showed that, the strain entering into the exponential growth phase needed about 30 hours, and the oxidation rate was more than 99% after 60 hours. The pH value increased from 2.08 to 2.64 at first 30 hours and then decreased to 2.0, and finally maintained at 2.0 in the whole growth process. The isolated strain provided a potential alternative method to handle the steel-picking sulfuric acid waste liquor.
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