Abstract
Phosphate-solubilizing bacteria (PSB) function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Isolation and application of salt-tolerant or halophilic PSB will facilitate the development of saline-alkali soil-based agriculture. A moderately halophilic bacterium was isolated from the sediment of Daqiao saltern on the eastern coast of China, which also performs phosphate-solubilizing ability. The bacterium was assigned to genus Kushneria according to its 16S rRNA gene sequence, and accordingly named as Kushneria sp. YCWA18. The fastest growth was observed when the culturing temperature was 28°C and the concentration of NaCl was 6% (w/v). It was founds that the bacterium can survive at a concentration of NaCl up to 20%. At the optimum condition, the bacterium solubilized 283.16 μg/mL phosphorus in 11 days after being inoculated in 200 mL Ca3(PO4)2 containing liquid medium, and 47.52 μg/mL phosphorus in 8 days after being inoculated in 200 mL lecithin-containing liquid medium. The growth of the bacterium was concomitant with a significant decrease of acidity of the medium.
Highlights
Phosphorus (P) is one of the major essential macronutrients for plants, which is applied to the soil in the form of phosphatic manure
Soil microorganisms involve in a wide range of biological processes including the transformation of soil phosphorus
The modified Ca3(PO4)2 culture medium contained with the following ingredients (l−1) [9]: glucose 10 g, (NH4)2SO4 0.5 g, NaCl 30 g, KCl 0.3 g, FeSO4·7H2O 0.03 g, MnSO4·4H2O 0.03 g, MgSO4·7H2O 0.3 g, Ca3(PO4)2 10 g, agar 20 g, H20 1000 mL, pH 7.0–7.5
Summary
Phosphorus (P) is one of the major essential macronutrients for plants, which is applied to the soil in the form of phosphatic manure. Saline-alkali soil-based agriculture develops quickly in recent years. Similar to the fertile soil-based agriculture, the intensive culturing of salt-tolerant and even saltresistant plants has dramatically decreased the availability of phosphorus in saline-alkali soil. Soil microorganisms involve in a wide range of biological processes including the transformation of soil phosphorus. They solubilize soil phosphorus for the growth of plants [4]. The growth of phosphate-solubilizing bacteria (PSB) often causes soil acidification, playing a key role in phosphorus solubilization [5]. Most PSB isolated previously performed relatively low salinity tolerance, being less appropriate for saline-alkali soil-based agriculture. It is urgently needed to isolate highly halophilic PSB for the development of salinealkali soil-based agriculture. A moderately halophilic, phosphate-solubilizing bacterium YCWA18 was isolated and characterized
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