Abstract
A radioactive peptide has been isolated from rat liver mitochondria after labeling with leucine-C14 in vivo in the presence of cycloheximide. It has the following properties: 1. It is extremely hydrophobic; 2. After extraction from the membrane, it is tightly bound to phosphatides, possibly by ionic forces; 3. It gives a ninhydrin positive reaction; 4. Only one labeled compound with a molecular weight of about 2000 was detected; 5. It is not hydrolyzable by cone. HCl, but is split by subtilisin; 6. It contains most of the main amino acids.
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More From: Biochemical and Biophysical Research Communications
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