Isolation and characterization of a pathogenesis-related protein 1 (SlPR1) gene with induced expression in tomato (Solanum lycopersicum) during Ralstonia solanacearum infection

Abstract

In order to explore the function of pathogenesis-related (PR) proteins in regulating tomato (Solanum lycopersicum) biological stress response, a PR protein gene (SlPR1) (Gen ID: Solyc01g106620.2) was isolated from tomato by RT-PCR. The full-length cDNA was 760 bp, which encoded a total of 179 amino acids. The cDNA contained a 42 bp 5′ non-coding region, a 178 bp 3′ non-coding region, and an open reading frame (ORF) of 540 bp. Homologous sequence alignment and phylogenetic analysis indicated that SlPR1 was highly homologous with a S. tuberosum PR1 protein, followed by S. pennellii. The predicted molecular weight of SlPR1 was 20,123.47 Da, the isoelectric point was 8.48, and the protein was found to be a secreted protein with a transmembrane structure. Quantitative real-time PCR (qRT-PCR) revealed that SlPR1 gene expression was highest in tomato stems, and could be induced by infection with Ralstonia solanacearum, and treatment with salicylic acid (SA) and methyl jasmonate acid (MeJA).Virus-induced gene silencing (VIGS) of SlPR1 decreased plant resistance to bacterial wilt, suggesting that SlPR1 positively regulates tomato resistance to this disease.This study provides a reference for the further exploration of the role of SlPR1 in the response of tomato to bacterial wilt and other stressors.