Abstract
This paper reports the successful isolation and characterization of a novel phenol-degrading bacterial strain WUST-C1 from activated sludge. WUST-C1 is an aerobic, Gram-negative, nonsporulating, and short-rod or peanut-shaped bacterium, which occurs singly, in pairs, but mainly in clusters. 16S rDNA sequence alignment demonstrated this strain belonged to the genus of pseudomonas, with a 99.9% identity to 16S rDNA sequences of Pseudomonas aeruginosa EU170480. It could mineralize up to 1200 mg L–1 phenol within 36 h (99%). Besides phenol, WUST-C1 could grow aerobically on pyrocatechol, alpha-naphthol, hydroquinone, naphthalene, isoquinoline, and indole. WUST-C1 was resistant to ampicillin and chloromycetin. The growth kinetics of strain WUST-C1 on phenol as a sole carbon and energy source at 35 °C can be described using Haldane equation. It has a maximum specific growth rate (μmax) of 2.47 h–1, a half-saturation constant (Ks) of 48.7 mg L–1, and a substrate inhibition constant (Ki) of 100.6 mg L–1 with a R2...
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